r/oddlysatisfying Jun 30 '22 Shocked 2 Wholesome 4 Gold 2 Helpful 9 Silver 5

Removing Chlorophyll from a leaf.

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69.4k Upvotes

4.1k

u/James324285241990 Jun 30 '22 edited Jun 30 '22

That's actually "cellular scrubbing" or decellularization.

There's nothing left but the cellulose that makes up the structure of the leaf

866

u/issavoiddd Jun 30 '22

can you theoretically do this to a human?

1.4k

u/[deleted] Jun 30 '22 edited Jul 01 '22

[removed] — view removed comment

1.8k

u/Windbelow616 Jun 30 '22 Gold Wholesome Doom

Can we still try?

607

u/Applied_Mathematics Jun 30 '22

Sure, champ!

308

u/Ta2whitey Jun 30 '22

Can we start with the SCOTUS?

257

u/FisterRobotOh Refreshingly Crisp Jun 30 '22 Silver Helpful Wholesome

The EPA can’t stop you

44

u/shockeroo Jun 30 '22

Take my free reward and get out of here.

5

u/No-Height2850 Jul 01 '22

I needed this thread today. Thank you all

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207

u/Crabjock Jun 30 '22 Wholesome

You got spirit, kid.

Hey! Let's see if we can remove that!

53

u/DaveTheDog027 Jun 30 '22

gestures at the world broadly

That should do it

14

u/gulrurahof Jun 30 '22

Spiritless husk here. Can confirm

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u/Joe4o2 Jun 30 '22

Is this a Cave Johnson-ism?

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u/James324285241990 Jun 30 '22 edited Jun 30 '22

You sorta can wash out animal tissue, and just leave a framework. But it depends on the tissue. Chunk of muscle? No. Trachea? Sure. Bone? Certainly.

Edit, I stand corrected. You can apparently wash out any tissue

23

u/ghandi3737 Jun 30 '22

They can do muscle, I posted a picture of it up above. It's called decellurization, it leaves the collagen behind which acts as a framework for stem cells to grow onto.

They can PRINT a working (simple) muscle with a modified inkjet type printer loaded with amino acids (IIRC that's what was in the cartridges). Although I can't find this particular video (it was just a tube shaped muscle, very simple).

Here's a Ted talk about printing muscles. We are very fucking close to be able to make actual working organs that will not be rejected by the patient (hopefully, shhhh send them money for research).

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u/idbanthat Jun 30 '22

38

u/doblitos Jun 30 '22

That fetus looks like he's up to something.

13

u/somewhat-helpful Jun 30 '22

Lmfaooooo bro

7

u/Funkit Jun 30 '22

It’s red skull. We need captain America.

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u/scpclr5tz Jun 30 '22

Why tf is that for sale?! Why is it sold out?! Im on a list now for clicking that link aren’t I?

14

u/vyrelis Jun 30 '22

Sold out because there was very likely only the one. There's probably thousands of people who would buy something like this

11

u/darkest_irish_lass Jun 30 '22 edited Jun 30 '22

Sold out, too. Wtf, who drops 5K on something like this? I'm assuming a med student doing graduate work or someone doing medical research.

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u/archelon2001 Jun 30 '22

Yes, you could. The process is called decellularization. There is a structure called the extracellular matrix (ECM) which is a scaffold that surrounds the cells. In plants, this is made of cellulose and lignin; in animals, this is made of collagen. Decellularization takes out the cells and leaves the ECM behind. There's research being done into taking organs, removing the cells, and then reseeding the ECM with cells from a patient's own body to use in organ transplants. This would potentially alleviate the transplant shortage since you could use any heart, not just one from a donor that is blood type compatible, or even from other animals such as pigs, as well as eliminate the need for immunosuppressants. https://www.cleveland.com/healthfit/2012/08/ghost_heart_a_framework_for_gr.html

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u/dropkickpa Jun 30 '22

Dr. Taylor is on the cutting edge of this for hearts. It's insanely exciting stuff! https://www.cnn.com/2022/06/01/health/ghost-heart-life-itself-wellness/index.html

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u/AmbrGlw Jun 30 '22

Well, they'd die buuuuuut

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u/Djadelaney Jun 30 '22

We don't have cellulose skeletons, no. Animal and plant cells are different, plant cells have walls which are cellulose and animal cells are just squishy and membranous. I can't imagine animal cells would survive this treatment like the cellulose does. Humans have our own kind of cool skeletons anyway

35

u/DudeInThePurpleJeans Jun 30 '22

You can decellurise animal tissue to leave behind nothing but a scaffold on which to grow other cells. They're attempting to use pig hearts in this way to grow the patients cells on to, to get a heart that can be transplanted with fewer complications.

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u/TyrellCo Jun 30 '22

Might be this similar process called decellularization https://youtu.be/pd3TFB0wOI0. A protein scaffold is what’s left.

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u/boo_boo_technician Jun 30 '22

Cool. But why? What purpose does it serve?

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u/Zycosi Jun 30 '22

There's actually a company that is going through FDA trials right now using implants of decellularized asparagus filled with stem cells to repair spinal damage. No, I'm not joking.

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u/bmild-minus Jun 30 '22 edited Jun 30 '22

It makes it easier to see the structure of the leaf, or from what if seen trying to make meat out of this. Using the leftover structure for meat to grow in, so lab-grown meat has texture.

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5.0k

u/TheDeftEft Jun 30 '22

Chlorophyll? Shit, they took out every bit of living tissue.

2.4k

u/AndurielsShadow Jun 30 '22

Right? That's like saying "tearing out old carpet" and the video is of a tornado that ripped the whole house away except for a toilet and the tp roll. I mean, it's technically correct, but holy hell.

533

u/greyrobot6 Jun 30 '22

I’m very sleep deprived and my brain kept reading that as tomato. It ripped the whole house away. I need a nap

143

u/TwoKeezPlusMz Jun 30 '22

Ever see Attack of the Killer Tomatoes?

48

u/heelstoo Jun 30 '22

Say, Timmy, do you like movies about gladiators?

15

u/Sinthetick Jun 30 '22

Have you ever seen a grown man naked?

8

u/pinkkittenfur Jun 30 '22

Have you ever been in a Turkish prison?

10

u/StalyCelticStu Jun 30 '22

I picked the wrong week to give up glue-sniffing.

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u/wendyrx37 Jun 30 '22

When we were kids my parents would make us hats out of newspaper & give us the salt shaker & a fork to defend ourselves & then let us watch Attack of the killer tomatoes.

My family is weird.

5

u/TwoKeezPlusMz Jun 30 '22

Your family is awesome!

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u/Content-Positive4776 Jun 30 '22

But. Did they perform as advertised?

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u/Echolama Jun 30 '22 Gold

Chlorophyll? More like Borophyll!

104

u/mezzolith Jun 30 '22

NO, I WILL NOT MAKE OUT WITH YOU.

43

u/turdferguson_md1 Jun 30 '22

No milk will ever be our milk

17

u/Turdferguson586 Jun 30 '22

That Veronica Vaughn is one piece of ace.

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u/AlexJonesWasRight69 Jun 30 '22

Veronica Vaughan

13

u/turdferguson_md1 Jun 30 '22

So hot… etc etc ect

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u/ShaddapDH Jun 30 '22

YOU GOT BOROPHYLL MAN UP THERE TALKIN ABOUT GOD KNOWS WHAT AND ALL SHE'S TALKIN ABOUT IS MAKING OUT WITH ME! I'M HERE TO LEARN EVERYBODY, NOT MAKE OUT WITH YOU! GO ON WITH THE CHLOROPHYLL!

130

u/19mine Jun 30 '22

Sir, what you've just commented is one of the most insanely idiotic things I have ever heard. At no point in your rambling, incoherent comment were you even close to anything that could be considered a rational thought. Everyone on this thread is now dumber for having read it. I award you no upvotes, and may God have mercy on your soul.

53

u/FullMarksCuisine Jun 30 '22

Still one of my favorite lines ever written for cinema

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u/natesdiggittydog Jun 30 '22

A simple no would have sufficed ….

4

u/TorpleFunder Jun 30 '22

What's that from?

13

u/GammaEspeon Jun 30 '22

Billy Madison

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u/AnusNAndy Jun 30 '22

For the last 23 years I haven't been able to hear or see the world chlorophyll without hearing Billy's voice in my head.

13

u/tidesoncrim Jun 30 '22

He called the shit poop.

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u/simpledsp Jun 30 '22

That’s what I came to the comments to see!

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u/Handeatingcat Jun 30 '22

No I will not make out with you!

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u/Prince_Havarti Jun 30 '22 I'll Drink to That

Chlorophyll?, more like Borophyll.

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u/wolverine_553 Jun 30 '22

theres a medical institute thats working on making the technology to use the plant stem cells to make human organs, thus being able to jave more successful organ transplants. we can literally start growing human organs, although ots still in development. still cool af

36

u/Gonzobot Jun 30 '22

we can already grow organs using normal stem cells, why they gotta go after plants like that?

60

u/rubermnkey Jun 30 '22

the plants cells just act as a scaffold, they can't reproduce human cells using plant stem cells. they help provide structure while the organ develops, they aren't going to give you a soy kidney.

21

u/Axlfire Jun 30 '22

So... I can't have a pepper lung?

22

u/TwoKeezPlusMz Jun 30 '22

Keep smoking, you'll get there.

4

u/Axlfire Jun 30 '22

I want a pepper not a train

However...

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u/AnApexPredator Jun 30 '22

That sucks. I was hoping the eggplant emoji could finally be an accurate representation of my penis.

10

u/Strawbuddy Jun 30 '22

The dawn of the true soy boi

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u/TheDeftEft Jun 30 '22

Fewer ethical, religious, and financial issues.

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u/AndChewBubblegum Jun 30 '22

There's no ethical or religious problem with iPSCs, that thinking is a holdover from fetal stem cells, which have been supplanted in almost all research contexts. IPSCs are derived from adult humans, and patients can have them derived from their own tissues, making the chance for rejection basically zero.

There are a ton of research problems to solve before iPSC derived therapies become mature, but the hurdles are not the same as the older work done with fetal stem cells.

15

u/azra3l Jun 30 '22

BuT MuH GmO!

people are fucking stupid.

7

u/ZestyUrethra Jun 30 '22

Imo there are ethical issues around ipscs similar to crispr therapy - it'll be really really expensive for a long time but fundamentally change the nature of the care we can provide. It will be a challenge to provide that care equitably.

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u/wolverine_553 Jun 30 '22

because its probably cost effective idk

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u/RogerOverUnderDunn Jun 30 '22

the biggest hurdle though is they cant get the human body to take a plant into its system and not immediately destroy it, even immuno suppression drugs dont help at all. ad to be honest finding is drying up for the project. And old colleague of mine works for the same company.

they have almost totally moved on to using plant stem cells in cosmetics to hide aging in skin. However some neat things that happened are we discovered that one part of the plant stem cell is responsible for growth and reproduction of cells, if they can figure out a way to use this growth factor, to grow lab grown human stem cells, we may be able to grow our own replacement organs in a lab someday.

3

u/wolverine_553 Jun 30 '22

when you know we're gonna start saving people:

3

u/Beard_o_Bees Jun 30 '22

If we could coax a plant into growing into the shape of an ear, say.. that could be huge as you could then first apply a process similar to the one shown here to the plant and then use it as a 'scaffolding' for regrowing cartilage.

We're getting pretty good at growing different types of tissue, getting them into the proper shapes and structures is very difficult.

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u/Ctowncreek Jun 30 '22

Yeah this was removal of cells and leaving behind the cellulose/lignin in the veins.

Removing the chlorophyll only requires boiling the leaves in ethanol. It will definitely destroy the cells too, disrupting membranes to release the pigment, but its only one step.

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u/garrettj100 Jun 30 '22

Chlorophyll? MORE LIKE BORE-OPHYLL, AMIRITE?!?

(I'll see myself out.)

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u/-DrToboggan- Jun 30 '22

More like Borophyll!

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u/Bear-Necessities- Jun 30 '22

Think it's some form of iodine solution. My biology teacher in high school did this once but that was many years ago

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u/WateryTart_ndSword Jun 30 '22

For some reason I expected the collection of the chlorophyll to be the goal, rather than making the leaf transparent…

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u/wrenway Jun 30 '22 Today I Learned

the clear cellulose remnants of the leaf are usually the goal with this process.

scientists remove the chlorophyll from spinach leaves, sterilize them, then plant human heart cells on the leaves and pump both cells that line human veins as well as a solution mimicking human blood through the “veins” in the leaf to create a scaffold that will grow pseudo-heart muscle complete with a vascular system.

https://www.wpi.edu/news/wpi-team-grows-heart-tissue-spinach-leaves

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u/finntana Jun 30 '22

Absolutely amazing. Thank you for sharing.

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u/ImAJewhawk Jun 30 '22

Just for clarification, that was a proof of concept, not something that’s regularly done.

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u/ploploploploplopped Jun 30 '22

I was 100% sure you were just making that up in a super detailed elaborate joke. Science has gone too far

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u/DownsonJerome Jun 30 '22 I'll Drink to That

Science has not gone far enough

78

u/elliiot Jun 30 '22

👍👍 Science should be transplanting the chlorophyll to my skin and grafting those leaves directly to my heart

21

u/Astilaroth Jun 30 '22

Oh god now I want leaves. How cool would that be. Although we have a pet rabbit that roams the house freely so it shouldn't be too attractive to herbivores ...

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u/Arya_the_Gamer Jun 30 '22

Yeah we still don't have catgirls

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u/OMGlookatthatrooster Jun 30 '22

I'll take a spinach heart any day!

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u/[deleted] Jun 30 '22

But wait, there's more!

Did you know the CIA began experimenting on animal/machine hybrids to conduct surveillance in the 1960s? https://en.wikipedia.org/wiki/Acoustic_Kitty

While the single cat/cyborg hybrid they produced was killed by a passing car on its first mission that didn't stop them there! Someone realized planting electrodes in the brains of insects allowed them to control things like flapping the insect's wings faster, slower, stopping wings from beating entirely, even being able to control when the insects would change direction left or right.

The best part? If you implant the electronic hardware into the insect while it's undergoing metamorphosis in a cocoon or similar stage of its lifecycle, the insect will shift its internal organs around to make room for the listening devices, electrodes, batteries and other equipment! A visually identical bug to any other you might see outside, but inside it's a cyborg! And that's just the beginning..

https://law.ucdavis.edu/sites/g/files/dgvnsk10866/files/media/documents/Cyber-Insect-Drones-Heraclio-Pimentel-Jr.--Fall-2017.pdf

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u/jellosquare Jun 30 '22

Cool thing to hear about today. Good person.

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u/Potential_Regret_169 Jun 30 '22

Yup, expecting the same

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u/Kizik Jun 30 '22

Yeah, their goal here should have been made more... clear.

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u/airjordan77lt Jun 30 '22

Get outta here with that 😂

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u/sierramisted1 Jun 30 '22

hey, just because you didn’t like the joke doesn’t mean they have to leaf!

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u/airjordan77lt Jun 30 '22

please see yourself outta here too! Too many punny people on Reddit this morning Lolol

10

u/Spare-Blacksmith1376 Jun 30 '22

Great work everyone. Take the rest of the day off

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u/Jwhitx Jun 30 '22

The person making the video had different goals than whoever (or whomever....or whomstsoever) created the post title. Get it together, title makers of the world. Cmon!

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u/ButteredBabyBrains Jun 30 '22

Do you have a link to the original?

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u/Chaosmusic Jun 30 '22

Well the second video is them using the chlorophyll to make horrifying plant/animal mutants but they didn't consider that appropriate for oddly satisfying.

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u/Joe_d_d Jun 30 '22

I suppose they could now recellularize with human tissue and make a leaf-man.

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u/RalseiXD Jun 30 '22 Helpful

"i believe i made myself clear"

232

u/ChefMork Jun 30 '22

I beleaf you missed an opportunity

53

u/RalseiXD Jun 30 '22

fuck, should've thought of that

15

u/StrokeGameHusky Jun 30 '22

Where’s the pun?

oh there it is

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u/SandStorm4078 Jun 30 '22

"I must be honest with you. Until this point, I have not been fully transparent."

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u/skuIIdouggery Jun 30 '22

From the reaction of the water and the powder (and also the container that the powder came from), it looks like this person mixed water into a highly basic substance like sodium hydroxide.

 

PSA: Do NOT mix water directly into whatever substance you're trying to make a solution out of, especially if the substance reacts in this way. Measure out the amount of water you need, and then slowly add the substance into that.

 

Source: I'm a dumbass who's picked up chemical burns from bad science'ing.

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u/rook_armor_pls Jun 30 '22

Especially important when mixing, or diluting concentrated acids. Had a mate nearly covering his face in sulfuric acid because of this fuckup.

The reason for this (in case anyone is interested) is that the dilution process of these substances is extremely exothermic and water will start boiling off immediately when added to the acid, pulling the acid with it in a quite explosive manner.

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u/Pale_Reason4904 Jun 30 '22 Silver Gold Helpful Duck Dance

Chlorophyll more like boraphyll

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u/CantBake4Shit Jun 30 '22

No I won't make out with you!

53

u/trynot2screwitup Jun 30 '22

Mr. Madison, what you've just said is one of the most insanely idiotic things I have ever heard. At no point in your rambling, incoherent response were you even close to anything that could be considered a rational thought. Everyone in this room is now dumber for having listened to it. I award you no points, and may God have mercy on your soul.

12

u/CantBake4Shit Jun 30 '22

Do you know that by memory or did you look it up. I must know.

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u/BurnerForJustTwice Jun 30 '22

Hey look everybody, Billy peed his pants.

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u/Joedirt6705 Jun 30 '22

If peeing in your pants is cool, consider me Miles Davis.

63

u/Unchristian30 Jun 30 '22

That’s the grossest thing I’ve ever heard! Everyone on the bus!

58

u/CrookedRocket Jun 30 '22

NO YELLING ON THE BUS!!!!!!!!!!

24

u/skttrbrain1984 Jun 30 '22

That Veronica Vaughn is one fine piece of ayshe. I know from experience, if you know what I mean.

15

u/Britrae87 Jun 30 '22

No you don’t.

8

u/kadno Jun 30 '22

Well, not me personally, but a guy I know. Him and her GOT IT ON WHOOOEEEE

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u/UltraLobsterMan Jun 30 '22

O’DOYLE RULEZ!

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u/CantBake4Shit Jun 30 '22

No milk will ever be our milk.

33

u/_fly-on-the-wall_ Jun 30 '22

sooo hot! want to touch the hiney!

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u/420Chopin Jun 30 '22

Who would steal 30 bagged lunches?

3

u/brianfine Jun 30 '22

Touch her boob? That’s assault, brother.

Do you double dare me?

14

u/littlebuck2007 Jun 30 '22

You got Chlorophyll Man up there talking about God knows what, and all she can talk about is making out with me!

8

u/DrDiarrheaBrown Jun 30 '22

Go on with the chlorophyll!

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u/1i_lu Jun 30 '22

This is the only reason I came to the comments, thank you

22

u/enormuschwanzstucker Jun 30 '22

How about you, Sideburns? You want some of this milk?

11

u/EmperorSexy Jun 30 '22

I’d rather have a beer

10

u/anxessed Jun 30 '22

Miss Lippy’s car… is GREEN.

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u/ItchySnatch69 Jun 30 '22

It's way too hot for a penguin to be running around out here

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u/NewFuturist Jun 30 '22

Stop looking at me swan!

10

u/Alex_Keaton Jun 30 '22

Mr. Madison, what you've just said is one of the most insanely idiotic things I have ever heard. At no point in your rambling, incoherent response were you even close to anything that could be considered a rational thought. Everyone in this room is now dumber for having listened to it. I award you no points, and may God have mercy on your soul.

7

u/gyarnar Jun 30 '22

Okay, a simple "wrong" would've done just fine.

43

u/I_Drink_My_Own_Urine Jun 30 '22

Yeah i'm gonna make like a tree, and get the hell outta here

17

u/beardingmesoftly Jun 30 '22

People in glass houses sink ships!

8

u/I_Drink_My_Own_Urine Jun 30 '22

Loose lips shouldn't throw stones!

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u/Tag82 Jun 30 '22

There it is! I was looking for this.

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u/vicvega88 Jun 30 '22

DAT IZ CO-RRECT

5

u/Gniv1031 Jun 30 '22

Came specifically for this comment - unsure why it’s not the top

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u/gyarnar Jun 30 '22

I can't hear that word without thinking of that.

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u/Terrible_Feature-532 Jun 30 '22

I'm such an idiot. At first glance I read: Removing Chernobyl from a leaf.

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u/qisope Jun 30 '22

well, if there was any Chernobyl in there it's probably gone now

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u/riyadhelalami Jun 30 '22

Hey, exactly this happened to me. Wow

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u/DudeMcBraski Jun 30 '22

Thank god I’m not the only one

9

u/pizza_for_nunchucks Jun 30 '22

That makes at least one of us.

3

u/octobertwins Jun 30 '22

I'm glad you said that because this has been happening to me a lot lately.

I don't think it makes us idiots. I do feel a little worried that it is happening so often.

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u/everydayasl Jun 30 '22

But why?

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u/LifeIzBeautiful Jun 30 '22

Skeletonized leaves are often coated in metal and used in jewelry (for smaller ones) or as ornaments or you can spray paint them or modpodge them and use them in crafts.

160

u/MadLintElf Jun 30 '22

Thank you, I'd love to give it a shot.

Found a link to the process if anyone is interested.

https://www.instructables.com/Leaf-Skeletonization/

55

u/hlight Jun 30 '22

Thanks! I'm looking on ingredients photo. Is cat really necessary to this?

56

u/MadLintElf Jun 30 '22

The cat is optional, but always nice to have :)

26

u/hlight Jun 30 '22

Oh, now I see that it's only as supervisor. I was afraid beacuse you know, cat is "one use" ;)

10

u/CaptainLollygag Jun 30 '22

No, cats are required to approve of everything. Thankfully I have 4 judgmental fuzzy ones here.

4

u/MadLintElf Jun 30 '22

I have one, and unfortunately for him my 3 parrots are the one's that judge him. They don't hurt him, just scream at him when he's near the cage or begging for our food (they want some too).

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u/bengalese Jun 30 '22

Might want to keep the cat away from all those toxic liquids.

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u/MadLintElf Jun 30 '22

Definitely a good idea.

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u/marky755 Jun 30 '22

No. Way. I always thought these were just moulds with plastic or metal poured in.

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u/ockhams-razor Jun 30 '22

You can spray paint them green and make them look like a leaf!

10

u/scoopzthepoopz Jun 30 '22

The circle of lifeeeeee

4

u/TheHYPO Jun 30 '22

Is there a reason you can't just do this with an 'as is' leaf?

13

u/LifeIzBeautiful Jun 30 '22

You can do some things with them, but a lot of the beauty in the pieces are seen as being in the vein structure, which is more prominent if they've been treated.

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u/jenyto Jun 30 '22

I assume that when the leftover tissue rot, it might make some gas and buldge and ruin the coating.

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u/______V______ Jun 30 '22

TL/DR: it has uses in the medical field.

Some bioengineers did basically the same thing but with a spinach leaf because they noticed how its “blood vessels” were almost identical to the ones we have in our hearts. From that they opened the way for spinach-based heart tissue repairs. It is more complicated than this, heard about this on an episode of Bill Nye saves the world on Netflix

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u/HudLichen Jun 30 '22

They can also just be flattened and dried up to be used as bookmarks.

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u/amber0100 Jun 30 '22

Can i ask for the chemicals were used in that vid?

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u/LordofAlkanes Jun 30 '22

In one experiment while I was studying chemistry we'd use acetone to extract chlorophyll from parsley leaves.

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u/dimensionargentina Jun 30 '22

You can use alcohol just to extract.

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u/Zipzmahpantzup Jun 30 '22

Ok I’m drunk now what’s the next step

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u/Long-hair_Apathy Jun 30 '22

Instructions unclear, I am now transparent

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u/Substantial-Girth Jun 30 '22

Ask firmly, yet politely and the chlorophyll will leave.

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u/marasydnyjade Jun 30 '22

I have no idea what they used, but I’m pretty sure I remember that you can use rubbing alcohol - it just takes a while.

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u/testscicles Jun 30 '22

So is this how cocaine is made or something.

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u/ClifftonSmith Jun 30 '22

What's the purpose of diet leaves?

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u/cuddlefish143 Jun 30 '22

Cool! Now put it back in 😁

5

u/Ty-Dyed Jun 30 '22

Is this the plant equivalent of bleeding someone dry?

7

u/OfBooo5 Jun 30 '22

Not it’s chloroempty

4

u/[deleted] Jun 30 '22

I'd love a step-by-step on that. There are so many cool things one could do with that.

18

u/JVints Jun 30 '22

Really stripped a leaf of their clothes. Pandemic changed people man.

3

u/askingjaguar122 Jun 30 '22

That's cool never seen this done before.